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KMID : 1234920170170010001
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Journal of Korean Oriental Association for Study of Obesity
2017 Volume.17 No. 1 p.1 ~ p.9
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The Anti-Inflammatory Effect of Pulsatilla koreana Methanol Extract in Lipopolysaccharid-Exposed RAW 264.7 Cells
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Lee Gui-Sun
Kim Doo-Hee
Park Joong-Hyun
Choi Hee-Seung
Heo Seong-Kyu
Cha Yun-Yeop
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Abstract
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Objectives: This study was performed to identify the anti-inflammatory effect of Pulsatilla koreana (PK) methanol extract on lipopolysaccharide (LPS) induced inflammatory.
Methods: There were five groups. They were control group, LPS-exposed PK methanol extract group (0 μg/ml, 10 μg/ml, 30 μg/ml, 100 μg/ml). To measure out cytotoxicity of PK, we performed the MTT assay. To evaluate the anti-inflammatory effect of PK, we examined the inflammatory mediators, such as nitric oxide (NO), prostaglandin E2 (PGE2), and pro- inflammatory cytokines (interleukin [IL]-1β, IL-6, tumor necrosis factor-α [TNF-α], IL-10).
Results: 1. The extract of PK (∼100 μg/ml) itself did not have any cytotoxic effect in RAW 264.7 cells. 2. The concentration of plasma NO and PGE2 in PK methanol extract group showed a lower values than those of control group. 3. The concentration of plasma IL-1β, plasma IL-6, and plasma TNF-α in PK methanol extract group showed a lower values than those of control group. 4. The concentration of Plasma IL-10 in PK methanol extract groups showed higher than control group; however, these values showed no significantly different.
Conclusions: According to this study, the extract of PK could be used as a protective agent against inflammation.
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KEYWORD
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Pulsatilla koreana, Lipopolysaccharide, RAW264.7, Anti-inflammatory effect, Cytokines
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